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Some background:

I am building a confocal fluorescence microscope. Long story short, photons are recorded using a Time Correlated Single Photon Counter (TCSPC). This TCSPC is capable of time stamping each individual photon.

Since the detector is a point detector, I "scan" a laser beam over the sample using galvo mirrors to image it and I correlate the position of my laser beam with the photon data.

Laser scanning is achieved by generating a wave form on an NI-USB6343 (specs in link) AO channel. At the start of each wave form period I also generate a pulse on one of the digital outputs of the NI device. This digital out is recorded by the TCSPC on a dedicated channel and serves as a reference to reconstruct the images. For this, the TCSPC has a dedicated channel.

The issue

I can do programming and chemistry but have zero knowledge on electronics.

APDs, TCSPCs etc... all operate on 50 Ohms (no idea why). The NI box is not particularly designed for that it seems and any 5V triggers generated by it are not registered by the TCSPC.

Connecting the digital out of the NI box to a scope using a 50 Ohm BNC cable with a 50 Ohm terminator on a T-splitter, I can indeed see that my pulses are not 5 V anymore but merely 1.5 to 2 V. All other aspects of my pulses (rise time) seem to correspond to the TTL spec.

These are the relevant spect of the DO

NI DO specs

And this is the spec of the TCSPC:

TCSPC input spec

Finally, the specs of the detector (of which pulses are being picked up by the TCSPC):

Detector specs

Since the TCSPC costs more than a luxury sedan, I'd like to get some feedback on the type of circuitry that might help to adapt the output of the NI box to the requirements of the TCSPC without the risk of damaging either.

I would also be helped by potential suppliers of such equipment or perhaps companies where I could order custom circuits to achieve my goal.

I have looked at posts like this but given I have zero knowledge of electronics let alone soldering skills, I couldn't make anything out of the supplied information...

Edit

As suggested in the comments, the TTL specs look like the device might be intended to receive negative voltage signals (and detect falling edges). However, looking at the provided manual, there seems to be a distinction between the signal inputs (to which I connect detectors and which have high time resolution) and the "low resolution" trigger inputs.

In the manual I found a specific description referring to them:

Sync

So there is a reference to a NIM style connector (NIM seems to predate TTL with True−16 mA into 50 ohms = −0.8 volts) but it clearly says "TTL in". So I assume that those M1 to M4 channels should accept "standard" TTL, i.e. positive voltage signals.

Moreover, most movable stages or salvo scanners indeed provide real TTL triggers to indicate "start of line" or "start of frame" events while raster scanning an image area...

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  • \$\begingroup\$ "my pulses are not 5 V anymore but merely 1.5 to 2 V": is the VAR knob at the oscilloscope set to full scale? \$\endgroup\$
    – Curd
    Commented Jul 18, 2015 at 21:36
  • \$\begingroup\$ @Curd I cannot check right now but actually, the scope is a very entry level Tektronix and I do not remember it having a VAR switch. Nonetheless, removing the 50 Ohm terminator from the splitter restores the NI generated pulses to a 5V amplitude if that helps... \$\endgroup\$
    – Kris
    Commented Jul 18, 2015 at 21:46
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    \$\begingroup\$ Notice that the TCSPC specifies a input voltage range of 0 to negative 1V, and max range (damage level) of +/- 1.5V. The table makes it looks like that does apply to the trigger input. Given the TCSPC costs more than a luxury sedan, you may want to be certain about the signal requirements before experimenting more with the trigger signal. \$\endgroup\$
    – rioraxe
    Commented Jul 19, 2015 at 0:24
  • \$\begingroup\$ @rioraxe You are right to mention this. However, as in the amendment to my question, the manufacturers specs are at least somewhat confusing... \$\endgroup\$
    – Kris
    Commented Jul 19, 2015 at 8:02

2 Answers 2

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The NI box specs for Ioh are either 16 or 24 mA.

1 volt into a 50 ohm load requires 1/50, or 20 mA.

2 volts into a 50 ohm load requires 2/50 or 40 mA.

So you would expect your NI box to produce pulses on the order of 1.5 volts, since in general you'll get better than minimum output.

And this is exactly what you are getting.

Using standard TTL to drive 50 ohms is a problem which goes way back. I'd advise making a little box to go between the NI and TCSPC which uses a MOSFET driver and run it off 5 volts. Something like a MAX4426/4427. Connect the NI box to your buffer box directly (no coax, or as short as humanly possible - preferably make your buffer box with a female BNC connector on the input), and put about a 200 - 300 ohm resistor on the buffer input as a partial termination. If you don't like the results from a MOSFET driver, use a 74FCT244 with all 8 inputs tied together and all 8 outputs tied together. Use only a P output from the NI box.

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If the TCSPC expects TTL level input and yet internally terminated to 50 ohms. Then the following simple conversion should work:

schematic

simulate this circuit – Schematic created using CircuitLab

  • This relies on the TCSPC internal termination be a resistor to ground.
  • My guess is that the additional delay would be in the order of less than a few ns.
  • The amplitude of the high level output voltage would be reduced by Vbe of Q1 of approx 0.7V. That should still be fine if the NI Box output is 3.x V or higher.
  • The collector of the transistor needs to be powered by 5V. If the NI Box does not have a voltage output like that, but it has an USB port, then one way is to search and buy a "USB power cable" to get 5V.
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