I am curious what methods are most suitable for securing PCBs. I have a large PCB which is fixed to a sheet metal enclosure using #4-40 x 1/4" stainless steel screws. The receiving end is a #4-40 captive standoff (PEM product). Initially I was not doing anything to prevent the screws from vibrating out. Sure enough, I started getting complaints that the screws were backing out just during shipping alone. So, I started to use a loc-tite product. This works well, but it's very labor intensive to put a drop on each screw. I have not used a lock washer as it tears up the PCB. Any suggestions?
There are a bunch of options. You are encountering the "Quality costs money" paradigm that shows up in production. In production it is also super important to make decisions - not on how something seems but on actual measured results.
Perhaps brushing the heads of the screws with locktite seems labour intensive. But if you measure the time/cost for an assembler to do this it may be less expensive than your alternatives.
Now it sounds like you've got pem inserts pressed into bent metal. There are plastic inserts that have tangs for capturing the board. They have a wide base and press in through a hole in the metal and use tangs for stability on the metal side. The plus side: no screws or screw drivers. Low assembly cost. Down side: its hard to design them in after you've gone to production with metal pem insert design. Maybe next time.
Another option is self tapping screws into the metal with plastic tube standoffs. A bit tricky to assemble.
Another option is self tappers into plastic inserts. These are smaller profile than the snap-in inserts above, and still require screws and screw drivers. But you might get away with them after going to production.
Use self tappers where you can. They bite in for good retention. And anything threaded adds cost to the piece parts. Better yet - use snap-ins.
Torque spec. Use a torque screw driver when inserting the screws, whether pneumatic, electric, or hand driven. Figure out the best torque setting and use it. The screws shouldn't be backing out that much that often that early if they are torqued in.
All that: maybe a quick brush with locktite doesn't cost that much.
Remember: "Quality costs money."
You may also consider PEM SNAP-TOP Standoffs or similar? They install into steel metal and there are no loose parts to drop.
I personally err away from crinkle washers etc; they've been proved to be mostly useless and in some cases detrimental. See NASA Reference Publication 1228, Fastener Design Manual for more information.