You have an inverting amplifier, which means your gain is negative.
$$ A_V = \frac {V_{out}} {V_{in}} = - \frac {R_F} {R_2} $$
If you want positive gain, use a non-inverting amplifier.
$$ A_V = \frac {V_{out}} {V_{in}} = 1 + \frac {R_F} {R_2} $$
An op-amp is a directly-coupled amplifier, so it will amplify ac or DC signals.
If it is a sinewave, the negative gain of inverting amplifier means the output will be inverted 180°. It is still a sinewave.
Put it through a second inverting amplifier and the negatives will cancel out. If you are dealing with audio, you will need several stages to match input impedance of source (antenna - μW) and output impedance of load (8Ω speaker - W).
As a DC input signal, the output will be inverted. So a positive signal will make a negative signal and vice versa.
Positive or negative levels are just potentials. As Felthry says "Perhaps you want a strong and steady negative voltage."