I have two audio signals, A and A+B on two different wires. I would
like to know if there is a solution to electronically retrieve the B
signal alone.
You have a computer so download a free wave editor and input the two audio signals to your PC and save them as a stereo wave file. Next open the wave editor (I use Wavelab but it isn't free) and then open the wave file and use the tools you normally get to manipulate audio.
The easiest tool is one that inverts the A channel. Next, play the stereo file (with A inverted) as a mono file and you'll get B coming over your speakers.
Is there any IC or any kind of circuit that could allow me to do that
kind of substract ?
Well you don't need an IC other than the ones in your PC.