I want to program an AVR attiny2313 with my AVR ISP MKII

So I built a header that connects to a breadboard where I want to burn the firmware to the ATTiny via ISP,

so my question is,

I have a design that uses an external Crystal of 16MHz

but I don't have space on the actual protoboard for the ISP header,
so can i upload the firmware with out a Crystal [will configuration of fuses work?] and then use it the proper external Crystal ?