If you PCB is "standard", ie 1 or 2 layers, no ball grid array components, no very thin traces or clearances, rectangular shape, ... and if a "prototyping" quality is enough for you, then you have plenty of PCB assembly companies that offer very low prices (<50$ exclusing the cost of components). They are often based in china, and simply do several "standard" prototypes (for different clients) at once on the same PCB, and cut it afterwards.
If you don't require high reliability, nor ultra fast assembly, then it might be an interesting solution.
As soon as you start to have some specific requirements on the PCB itself or assembly (ie more than 2 layers, ball grid array, sizes bigger than usually 100*100mm, ...) prices increase, but often remain in the 50-200$ range.
You also get some similar prototyping services in many countries, which is a bit faster, but more expensive.
On the other hand, if you expect "industrial production" with full testing (X-ray, continuity test, isolation test, functional tests, on circuit programming of microcontolers, ...) for a single unit, then it will be very expansive for a single unit.
If you ask a manufacturer specialized in big series, then you will likely pay use setup costs (making the price very high for a single PCB).
So basically, if you want a single PCB (and don't plan a big series of the same wanting to order it from the same manufacturer), then make sure to choose a manufacturer specialized in prototypes. Then you choose between cheep manufacturing in China (with random quality), or expensive manufacturing locally.
PS : from your question, I would guess that you are not very used to have PCBs manufactured and assembled. So excepted if you are in a hurry (which you don't seem to be) or if you have very special requirements, I would start with a cheep assembly, in order to validate your design. If you have to modify something, you avoid paying twice the high price. If it is fine, then you can decide if the prototype is of good enough quality, or if you want to order a "final" version of better quality (this time, without doubts if the design is correct or not), the prototype the being a spare in case you get any issue with the new one.